Labprice APRIL Recombinant Protein

Description: APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, compete for two receptors, TACI and BCMA. ARPIL has the ability to stimulate proliferation of various tumor cell lines including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF like receptor binding of the APRIL precursor. Recombinant human APRIL is a soluble 16.3 kDa protein, consisting of 146 amino acid residues.

Tested Applications: N/A
Applications: N/A
Predicted Molecular Weight: N/A
Physical state: Lyophilized
Buffer: N/A
Concentration: N/A
NCBI official symbol: TNFSF13
Accession #: NP_001185551.1
Protein GI: 310750385
NCBI gene ID#: 8741
NCBI official full name: tumor necrosis factor (ligand) superfamily, member 13
NCBI organism: Homo sapiens
Peptide sequence: AVLTQKQKKQ HSVLHLVPIN ATSKDDSDVT EVMWQPALRR GRGLQAQGYG VRIQDAGVYL LYSQVLFQDV TFTMGQVVSR EGQGRQETLF RCIRSMPSHP DRAYNSCYSA GVFHLHQGDI LSVIIPRARA KLNLSPHGTF LGFVKL
SWISSPROT #: O75888
Background Reference 1: N/A
Background Reference 2: N/A
Background Reference 3: N/A
Background Reference 4: N/A
Background Reference 5: N/A
Source: High-5 Insect cells
Species: Human
By Source: Other
By Species: Human
Fusion tag: N/A
Sequence: AVLTQKQKKQ HSVLHLVPIN ATSKDDSDVT EVMWQPALRR GRGLQAQGYG VRIQDAGVYL LYSQVLFQDV TFTMGQVVSR EGQGRQETLF RCIRSMPSHP DRAYNSCYSA GVFHLHQGDI LSVIIPRARA KLNLSPHGTF LGFVKL
Biology activity: Determined by its ability to induce NF-?B activation in HEK293-NF-?B reporter cells transfected with BCMA receptor.
Purity: Greater than 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin level is less than 0.1 ng per µg (1EU/µg).

The lyophilized APRIL recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted APRIL is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing APRIL recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

This product is for research use only.