Labprice TSC2 Antibody

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Description: TSC2 Antibody: Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. At least three isoforms of TSC2 exist.

Research area: Signal Transduction
Host species: Rabbit
Species reactivity: Human, Mouse
Homology: Predicted species reactivity based on immunogen sequence: Rat: (86%)
Immunogen: TSC2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human TSC2.
The immunogen is located within amino acids 1450 - 1500 of TSC2.
Tested Applications: E, WB
Applications: TSC2 antibody can be used for the detection of TSC2 by Western blot at 1 - 2 μ
g/mL.
Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
Specificity: N/A
Positive control 1: Cat. No. 1284 - L1210 Cell Lysate
Positive control 2: Cat. No. 1205 - Jurkat Cell Lysate
Positive control 3: N/A
Positive control 4: N/A
Positive control 5: N/A
Positive control 6: N/A
Predicted molecular weight: Predicted: 200 kDa
Observed: 200 kDa
MW explanation: N/A
Validation: N/A
Isoforms: N/A
Purification: TSC2 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical state: Liquid
Buffer: TSC2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
NCBI official symbol: TSC2
Accession #: NP_000539
Protein GI #: 116256352
NCBI gene ID#: 7249
NCBI official full name: tuberous sclerosis 2
NCBI organism: Homo sapiens
SWISSPROT #: P49815

TSC2 antibody can be stored at 4˚
C for three months and -20˚
C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions for each application to be determined by the researcher.