Labprice ELISA kit for Goat IgG (Immunoglobulin G)

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G (IgG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin G (IgG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin G (IgG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
10nm. The concentration of Immunoglobulin G (IgG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Description: A sandwich ELISA kit for detection of Immunoglobulin G from Goat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Species reactivity: Goat
Sensitivity: 1.5ng/mL
Detection range: 3.91-250ng/mL
Standard solution concentration: 250ng/mL
ELISA type: Sandwich
Assay length: 3h

The Goat IgG ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.

This kit is intended for research use only. Do not use for diagnostics purposes.