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SHH Recombinant Protein
id: LP-40-188-0025mg

Labprice SHH Recombinant Protein

ProSci


Description: Members of the Hedgehog (Hh) family are highly conserved proteins which are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). The biologically active form of Hh molecules is obtained by autocatalytic cleavage of their precursor proteins and corresponds to approximately the N-terminal one half of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can substitute for each other in experimental systems. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

Tested Applications: N/A
Applications: N/A
Predicted Molecular Weight: N/A
Physical state: Lyophilized
Buffer: N/A
Concentration: N/A
NCBI official symbol: SHH
Accession #: NP_000184.1
Protein GI: 4506939
NCBI gene ID#: 6469
NCBI official full name: sonic hedgehog
NCBI organism: Homo sapiens
Peptide sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
SWISSPROT #: Q15465
Background Reference 1: N/A
Background Reference 2: N/A
Background Reference 3: N/A
Background Reference 4: N/A
Background Reference 5: N/A
Source: E. coli
Species: Human
By Source: E. Coli
By Species: Human
Fusion tag: N/A
Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
Biology activity: Biological activity was determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL - 226) cells. The expected ED50 for this effect is 0.8 - 1.0 µg/mL.
Purity: Greater than 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin level is less than 0.1 ng per µg (1EU/µg).

The lyophilized SHH recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted SHH is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing SHH recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

This product is for research use only.


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