Labprice APP Antibody

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Description: APP Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the brains of all suffers of Alzheimer's disease.

Research area: Neuroscience
Host species: Rabbit
Species reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Sheep: (100%), Rabbit: (100%), Guinea pig: (100%), Pig: (100%)
Immunogen: APP antibody was raised against a peptide corresponding to 10 amino acids of human amyloid A4 protein precursor (APP) corresponding to the amino terminus of the 4K Ab peptide generated by beta- and gamma-secretases.
The immunogen is located within amino acids 650 - 700 of APP.
Tested Applications: E, WB, IHC-P
Applications: APP antibody can be used for detection of APP and Ab peptide by Western blot at 1 μ
g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ
g/mL.
Antibody validated: Western Blot in human, mouse, and rat samples
Immunohistochemistry in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity: N/A
Positive control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive control 2: Cat. No. 10-301 - Human Brain Tissue Slide
Positive control 3: N/A
Positive control 4: N/A
Positive control 5: N/A
Positive control 6: N/A
Predicted molecular weight: Predicted: 83 kDa
Observed: 115 kDa
MW explanation: N/A
Validation: N/A
Isoforms: N/A
Purification: APP Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical state: Liquid
Buffer: APP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
NCBI official symbol: APP
Accession #: CAA30050
Protein GI #: 28721
NCBI gene ID#: 351
NCBI official full name: amyloid beta (A4) precursor protein
NCBI organism: Homo sapiens
SWISSPROT #: P05067

APP antibody can be stored at 4˚
C for three months and -20˚
C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions for each application to be determined by the researcher.