Labprice Bmf Antibody

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Description: Bmf Antibody: Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.

Research area: Apoptosis
Host species: Rabbit
Species reactivity: Human, Mouse
Homology: Predicted species reactivity based on immunogen sequence: Rat: (92%)
Immunogen: Bmf antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human Bmf.
The immunogen is located within the first 50 amino acids of Bmf.
Tested Applications: E, WB, ICC
Applications: Bmf antibody can be used for detection of Bmf by Western blot at 1 - 2 μ
g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ
g/mL. For immunofluorescence start at 5 μ
g/mL.
Antibody validated: Western Blot in human samples
Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity: N/A
Positive control 1: Cat. No. 1210 - HEK293 Cell Lysate
Positive control 2: Cat. No. 1211 - HepG2 Cell Lysate
Positive control 3: Cat. No. 1201 - HeLa Cell Lysate
Positive control 4: Cat. No. 17-001 - HeLa Cell Slide
Positive control 5: N/A
Positive control 6: N/A
Predicted molecular weight: Predicted: 20 kDa
Observed: 22 kDa
MW explanation: N/A
Validation: N/A
Isoforms: N/A
Purification: Bmf Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical state: Liquid
Buffer: Bmf Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
NCBI official symbol: BMF
Accession #: NP_277038
Protein GI #: 15723378
NCBI gene ID#: 90427
NCBI official full name: Bcl2 modifying factor
NCBI organism: Homo sapiens
SWISSPROT #: Q96LC9

Bmf antibody can be stored at 4˚
C for three months and -20˚
C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions for each application to be determined by the researcher.