Labprice ADAP Antibody

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Description: ADAP Antibody: The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-κB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.

Research area: Immunology
Host species: Rabbit
Species reactivity: Human, Mouse
Homology: N/A
Immunogen: ADAP antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human ADAP.
The immunogen is located within amino acids 640 - 690 of ADAP.
Tested Applications: E, WB, ICC, IF
Applications: ADAP antibody can be used for detection of ADAP by Western blot at 0.5 - 1 μ
g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ
g/mL. For immunofluorescence start at 20 μ
g/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity: N/A
Positive control 1: Cat. No. 1204 - K562 Cell Lysate
Positive control 2: Cat. No. 17-004 - K-562 Cell Slide
Positive control 3: N/A
Positive control 4: N/A
Positive control 5: N/A
Positive control 6: N/A
Predicted molecular weight: N/A
MW explanation: N/A
Validation: N/A
Isoforms: N/A
Purification: ADAP Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical state: Liquid
Buffer: ADAP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
NCBI official symbol: FYB
Accession #: NP_001456
Protein GI #: 42476118
NCBI gene ID#: 2533
NCBI official full name: FYN binding protein (FYB-120/130)
NCBI organism: Homo sapiens
SWISSPROT #: O15117

ADAP antibody can be stored at 4˚
C for three months and -20˚
C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions for each application to be determined by the researcher.