Labprice LASS5 Antibody

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Description: LASS5 Antibody: The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. LASS5, also called Trh4, is a 392 amino acid endoplasmic reticulum, multi-pass membrane protein. Functioning as a dihydro-ceramide synthase, LASS5 is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoyl-ceramide) in a fumonisin B1-independent manner. It uses palmitoyl-CoA as an acyl donor and is involved in the synthesis of C14, C16 and C18-ceramide. LASS5 is the most abundantly expressed and predominant ceramide synthase isoform in lung epithelia. Recent studies show that LASS5 partially correct growth and apoptosis.

Research area: Homeostasis
Host species: Rabbit
Species reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: LASS5 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human LASS5.
The immunogen is located within the last 50 amino acids of LASS5.
Tested Applications: E, WB, IF
Applications: LASS5 antibody can be used for detection of LASS5 by Western blot at 1 μ
g/mL. Antibody can also be used for immunoflourescence starting at 20 μ
g/mL. For immunofluorescence start at 20 μ
g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity: Multiple isoforms of LASS5 are known to exist. This antibody may cross-react with the highly homologous LASS6.
Positive control 1: Cat. No. 1220 - SK-N-SH Cell Lysate
Positive control 2: Cat. No. 10-301 - Human Brain Tissue Slide
Positive control 3: N/A
Positive control 4: N/A
Positive control 5: N/A
Positive control 6: N/A
Predicted molecular weight: N/A
MW explanation: N/A
Validation: N/A
Isoforms: N/A
Purification: LASS5 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical state: Liquid
Buffer: LASS5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
NCBI official symbol: CERS5
Accession #: NP_671723
Protein GI #: 22218345
NCBI gene ID#: 91012
NCBI official full name: ceramide synthase 5
NCBI organism: Homo sapiens
SWISSPROT #: Q8N5B7

LASS5 antibody can be stored at 4˚
C for three months and -20˚
C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions for each application to be determined by the researcher.