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MARCH8 Antibody
id: LP-4885-002mg

Labprice MARCH8 Antibody

ProSci

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Description: MARCH8 Antibody: MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.

Research area: Innate Immunity
Host species: Rabbit
Species reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (76%)
Immunogen: MARCH8 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MARCH8.
The immunogen is located within the last 50 amino acids of MARCH8.
Tested Applications: E, WB, ICC
Applications: MARCH8 antibody can be used for detection of MARCH8 by Western blot at 0.5 - 1 μ
g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ
g/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity: N/A
Positive control 1: Cat. No. 1201 - HeLa Cell Lysate
Positive control 2: Cat. No. 17-001 - HeLa Cell Slide
Positive control 3: N/A
Positive control 4: N/A
Positive control 5: N/A
Positive control 6: N/A
Predicted molecular weight: N/A
MW explanation: N/A
Validation: N/A
Isoforms: N/A
Purification: MARCH8 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical state: Liquid
Buffer: MARCH8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
NCBI official symbol: MARCH8
Accession #: NP_659458
Protein GI #: 50539410
NCBI gene ID#: 220972
NCBI official full name: membrane-associated ring finger (C3HC4) 8
NCBI organism: Homo sapiens
SWISSPROT #: Q5T0T0

MARCH8 antibody can be stored at 4˚
C for three months and -20˚
C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions for each application to be determined by the researcher.


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