Labprice GNPDA1 Antibody

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Description: GNPDA1 Antibody: Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA1 is one of two mammalian glucosamine-6-phosphate deaminases that are thought to have arisen though gene duplication, with the GNPDA2 protein closer in structure and activity to the E. coli enzyme. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2.

Research area: Homeostasis
Host species: Rabbit
Species reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen: GNPDA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GNPDA1.
The immunogen is located within the last 50 amino acids of GNPDA1.
Tested Applications: E, WB, ICC, IF
Applications: GNPDA1 antibody can be used for detection of GNPDA1 by Western blot at 1 - 2 μ
g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ
g/mL. For immunofluorescence start at 20 μ
g/mL.
Antibody validated: Western Blot in mouse samples
Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity: GNPDA1 is predicted to not cross-react with GNPDA2.
Positive control 1: Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive control 2: Cat. No. 17-010 - HEK293 Cell Slide
Positive control 3: N/A
Positive control 4: N/A
Positive control 5: N/A
Positive control 6: N/A
Predicted molecular weight: N/A
MW explanation: N/A
Validation: N/A
Isoforms: N/A
Purification: GNPDA1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical state: Liquid
Buffer: GNPDA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
NCBI official symbol: GNPDA1
Accession #: AAC05123
Protein GI #: 2935438
NCBI gene ID#: 10007
NCBI official full name: glucosamine-6-phosphate deaminase 1
NCBI organism: Homo sapiens
SWISSPROT #: P46926

GNPDA1 antibody can be stored at 4˚
C for three months and -20˚
C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions for each application to be determined by the researcher.