Labprice PCDH12 Antibody

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Description: PCDH12 Antibody: Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 12 (PCDH12) was initially identified through PCR screening of mouse heart microvascular endothelial cell RNA; further experiments revealed its mRNA to be strongly expressed in highly vascularized organs such as lung and kidney, in addition to glycogen-rich trophoblasts in the placenta. PCDH12-null mice are viable and fertile, but show reduced placental and embryonic sizes when compared to wild-type mice. Further studies showed significant expression changes in 2, 289 genes, including those involved in tissue morphogenesis, angiogenesis, cell-matrix adhesion and migration, immune response and chromatin remodeling.

Research area: Homeostasis
Host species: Rabbit
Species reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: PCDH12 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH12.
The immunogen is located within amino acids 30 - 80 of PCDH12.
Tested Applications: E, WB, IHC-P
Applications: PCDH12 antibody can be used for detection of PCDH12 by Western blot at 1 μ
g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ
g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Specificity: This antibody is predicted to not cross-react with PCDH18.
Positive control 1: Cat. No. 1204 - K562 Cell Lysate
Positive control 2: N/A
Positive control 3: N/A
Positive control 4: N/A
Positive control 5: N/A
Positive control 6: N/A
Predicted molecular weight: Predicted: 128 kDa
Observed: 125 kDa
MW explanation: N/A
Validation: N/A
Isoforms: N/A
Purification: PCDH12 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical state: Liquid
Buffer: PCDH12 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
NCBI official symbol: PCDH12
Accession #: NP_057664
Protein GI #: 7706113
NCBI gene ID#: 51294
NCBI official full name: protocadherin 12
NCBI organism: Homo sapiens
SWISSPROT #: Q9NPG4

PCDH12 antibody can be stored at 4˚
C for three months and -20˚
C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions for each application to be determined by the researcher.