Labprice FAM59A Antibody

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Description: FAM59A Antibody: FAM59A, also known as GAREM, was initially identified through a mass spectroscoptic method that measures different phosphotyrosine states of proteins in response to different cell stimuli. FAM59A is a downstream molecule in the EGF signaling pathway that is tyrosine-phosphorylated, and this phosphorylation is needed for the binding of Grb2, an adaptor protein crucial to the transduction of growth signals from the plasma membrane to the nucleus. ERK activation in response to EGF stimulation is regulated by FAM59A in COS-7 and HeLa cells, and the overexpression of FAM59A stimulated cell proliferation and colony formation in soft agar, suggesting that FAM59A might be a critical protein with roles in ligand-mediated signaling pathway of the EGF receptor and the tumorigenesis of cells.

Research area: Cancer
Host species: Rabbit
Species reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: FAM59A antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FAM59A.
The immunogen is located within amino acids 630 - 680 of FAM59A.
Tested Applications: E, WB, IHC-P, IF
Applications: FAM59A antibody can be used for detection of FAM59A by Western blot at 1 - 2 μ
g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ
g/mL. For immunofluorescence start at 20 μ
g/mL.
Antibody validated: Western Blot in rat samples
Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity: N/A
Positive control 1: Cat. No. 1464 - Rat Liver Tissue Lysate
Positive control 2: Cat. No. 10-301 - Human Brain Tissue Slide
Positive control 3: N/A
Positive control 4: N/A
Positive control 5: N/A
Positive control 6: N/A
Predicted molecular weight: N/A
MW explanation: N/A
Validation: N/A
Isoforms: N/A
Purification: FAM59A Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical state: Liquid
Buffer: FAM59A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
NCBI official symbol: FAM59A
Accession #: Q9H706
Protein GI #: 125991851
NCBI gene ID#: 64762
NCBI official full name: family with sequence similarity 59, member A
NCBI organism: Homo sapiens
SWISSPROT #: Q9H706

FAM59A antibody can be stored at 4˚
C for three months and -20˚
C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions for each application to be determined by the researcher.