Labprice ZNF536 Antibody

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Description: ZNF536 Antibody: ZNF536 is a recently identified zinc-finger protein that is expressed primarily in the developing nervous system and the cerebral cortex, hippocampus, and hypothalamus. ZNF536 possess ten zinc fingers and interacts with CtBP1, a corepressor for gene transcription. It is most closely related to transcriptional repressor ZNF219. Overexpression of ZNF536 in embryonic stem cells dramatically reduced the mRNA levels of neuronal marker genes such as Pax6, MAP2, and beta-tubulin III following retinoic acid (RA)-induced differentiation, while depletion of ZNF536 via RNAi resulted in elevated mRNA levels of these genes, indicating its role in inhibiting neuronal cell differentiation. Overexpression of RA receptor a rescues the inhibitory role of ZNF536, suggesting that ZNF536 might inhibit RA response element-mediated transcriptional activity.

Research area: Stem Cell
Host species: Rabbit
Species reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: ZNF536 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ZNF536.
The immunogen is located within amino acids 30 - 80 of ZNF536.
Tested Applications: E, WB, IHC-P, IF
Applications: ZNF536 antibody can be used for detection of ZNF536 by Western blot at 1 μ
g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ
g/mL. For immunofluorescence start at 20 μ
g/mL.
Antibody validated: Western Blot in human samples
Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity: N/A
Positive control 1: Cat. No. 1303 - Human Brain Tissue Lysate
Positive control 2: Cat. No. 10-301 - Human Brain Tissue Slide
Positive control 3: N/A
Positive control 4: N/A
Positive control 5: N/A
Positive control 6: N/A
Predicted molecular weight: N/A
MW explanation: N/A
Validation: N/A
Isoforms: N/A
Purification: ZNF536 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical state: Liquid
Buffer: ZNF536 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
NCBI official symbol: ZNF536
Accession #: NP_055532.1
Protein GI #: 7662092
NCBI gene ID#: 9745
NCBI official full name: zinc finger protein 536
NCBI organism: Homo sapiens
SWISSPROT #: O15090

ZNF536 antibody can be stored at 4˚
C for three months and -20˚
C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions for each application to be determined by the researcher.