Labprice PPAPDC1A Antibody

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Description: PPAPDC1A Antibody: Phosphatidate phosphatase (PAP) plays important role in lipid-signaling metabolism in eukaryotic cells. Two distinct types of PAP (PAP1 and PAP2) activity have been distinguished by their subcellular localization and differential sensitivity to N-ethylmaleimide(NEM) and Mg2+. A yeast diacylglycerol pyrophosphate (DGPP) phosphatase (DPP1) and mammalian DGPP phosphatase (PAP2) have been identified as Mg2+-independent and NEM-insensitive membrane-associated. PPAPDC1A (also known as DPPL2) and PPAPDC1B (DPPL1) form a novel type of Mg2+-independent and NEM-sensitive mammalian phosphatidate phosphatase showing broad substrate specificity. PPAPDC1A is preferentially expressed in endothelial cells. Studies of PPAPDC1A and PAP activity suggest that they may play a role in angiogenesis.

Research area: Signal Transduction
Host species: Rabbit
Species reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: PPAPDC1A antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human PPAPDC1A.
The immunogen is located within the last 50 amino acids of PPAPDC1A.
Tested Applications: E, WB, IF
Applications: PPAPDC1A antibody can be used for detection of PPAPDC1A by Western blot at 1 μ
g/mL. For immunofluorescence start at 20 μ
g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity: N/A
Positive control 1: Cat. No. 1303 - Human Brain Tissue Lysate
Positive control 2: Cat. No. 10-301 - Human Brain Tissue Slide
Positive control 3: N/A
Positive control 4: N/A
Positive control 5: N/A
Positive control 6: N/A
Predicted molecular weight: N/A
MW explanation: N/A
Validation: N/A
Isoforms: N/A
Purification: PPAPDC1A Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical state: Liquid
Buffer: PPAPDC1A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
NCBI official symbol: PPAPDC1A
Accession #: NP_001025230
Protein GI #: 73611920
NCBI gene ID#: 196051
NCBI official full name: phosphatidic acid phosphatase type 2 domain containing 1A
NCBI organism: Homo sapiens
SWISSPROT #: Q5VZY2

PPAPDC1A antibody can be stored at 4˚
C for three months and -20˚
C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions for each application to be determined by the researcher.