Labprice PPAPDC3 Antibody

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Description: PPAPDC3 Antibody: PPAPDC3, also known as nuclear envelope transmembrane protein 39 (NET39), was initially discovered in an in silico screen for secreted or membrane proteins. It is a member of the PAP2 superfamily of phosphatases and haloperoxidases. PPAPDC3 has recently been shown to act as a negative regulator of myoblast differentiation by diminishing the activity of the mammalian target of rapamycin TOR. PPAPDC3 is highly expressed in cardiac and skeletal muscle and becomes strongly upregulated during cultured myoblast differentiation tissues. Overexpression of PPAPDC3 in myoblasts repressed myogenesis while knockdown by RNA interference promoted differentiation indicating its part in the regulatory mechanism for myogenesis.

Research area: Signal Transduction
Host species: Rabbit
Species reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: PPAPDC3 antibody was raised against a 19 amino acid synthetic peptide from near the amino of terminus human PPAPDC3.
The immunogen is located within the first 50 amino acids of PPAPDC3.
Tested Applications: E, WB, IF
Applications: PPAPDC3 antibody can be used for detection of PPAPDC3 by Western blot at 1 - 2 μ
g/mL. For immunofluorescence start at 20 μ
g/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity: N/A
Positive control 1: Cat. No. 1401 - Mouse Heart Tissue Lysate
Positive control 2: N/A
Positive control 3: N/A
Positive control 4: N/A
Positive control 5: N/A
Positive control 6: N/A
Predicted molecular weight: N/A
MW explanation: N/A
Validation: N/A
Isoforms: N/A
Purification: PPAPDC3 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical state: Liquid
Buffer: PPAPDC3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
NCBI official symbol: PPAPDC3
Accession #: EAW87966
Protein GI #: 119608372
NCBI gene ID#: 84814
NCBI official full name: phosphatidic acid phosphatase type 2 domain containing 3
NCBI organism: Homo sapiens
SWISSPROT #: Q8NBV4

PPAPDC3 antibody can be stored at 4˚
C for three months and -20˚
C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions for each application to be determined by the researcher.