Labprice PDE2A Assay Kit

Description: The PDE2A Assay Kit is designed for identification of PDE2A inhibitors using_x000D_fluorescence polarization.The assay is based on the binding of a fluorescent nucleotide_x000D_monophosphate generated by PDE2A to the binding agent. PDE2A catalyzes the hydrolysis of_x000D_the phosphodiester bond in dye-labeled cyclic adenosine monophosphate (cAMP)._x000D_Nanoparticle beads selectively bind the phosphate group in the nucleotide product. This_x000D_increases the size of the nucleotide relative to unreacted cAMP. Since the degree of polarization_x000D_of a fluorophore is inversely related to its molecular rotation, dyes attached to the slowly-rotating_x000D_nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized_x000D_light. Conversely, dyes attached to the rapidly-rotating cyclic monophosphates will obtain_x000D_random orientations and emit light with low polarization. The key to the PDE2A Assay Kit is the specific_x000D_binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE2A_x000D_reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE2A1_x000D_for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in_x000D_fluorescent polarization that can then be measured using a fluorescence reader.