Labprice PD-1[Biotinylated]:PD-L1 Inhibitor Screening Colorimetric Assay Kit

Description: Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand_x000D_attenuates immune responses and is exploited by both tumors and viruses. The PD-_x000D_1[Biotinylated]:PD-L1 Inhibitor Colorimetric Screening Assay Kit is designed for screening_x000D_inhibitors of PD-1 receptor binding to PD-L1 ligand. This kit comes in a convenient 96-well_x000D_format, with biotin-labeled PD-1, purified PD-L1, streptavidin-labeled HRP, and assay buffer for_x000D_100 binding reactions. The key to this kit is the high affinity of biotin-labeled PD-1 for_x000D_streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First,_x000D_PD-L1 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L1 on the plate. Finally, the_x000D_plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to_x000D_produce color, which can then be measured using a UV/Vis spectrophotometer microplate_x000D_reader.