Anti-tdTomato, DyLight®488

Purification: Epitope affinity purified
Immunogen: Purified recombinant peptide produced in E. coli
Specificity test:In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).
Buffer: PBS, 20% glycerol and 0.05% sodium azide
References: Not yet referenced.

Description: Goat polyclonal antibody to tdTomato (red fluorescent protein) conjugated to DyLight® 488. tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.

Concentration: 2.5 mg/mL
Conjugation: DyLight®488
Clonality: Polyclonal
Istotype: IgGHost Species: goat
Confirmed Reactive Species: Reacts against tdTomato, mCherry, red fluorescent protein (DsRed) from so-called disc corals of Discosoma species.
Target Name: tdTomato, DyLight®488
Applications: WB, IF, IHC-F
Dilutions by application: WB:1:500-1:2,000, IF:1:50-1:500, IHC-F:1:50-1:1,000

Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

For research use only.