Human PLAP/ALPP (Placental Alkaline Phosphatase) CLIA KitElabscience Biotechnology
No significant cross-reactivity with analogues from other species was determined. Please, note that the data on cross-reactivity is limited. Other samples, aside from the tested ones, may be suitable to be used with this kit.
Description: This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PLAP/ALPP . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLAP/ALPP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLAP/ALPP , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PLAP/ALPP . You can calculate the concentration of Human PLAP/ALPP in the samples by comparing the RLU value of the samples to the standard curve.
Target antigen: PLAP/ALPP
Species reactivity: Human
Detection range: 31.25~2000pg/mL: Assay type: Sandwich
Detection method: Colormetric
Tested sample types: Serum, Plasma, Cell supernatant
Shipped on ice packs. Upon receipt store refrigerated at +4 deg. C.
For research use only.