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Human PLAP/ALPP(Placental Alkaline Phosphatase) ELISA Kit
id: LP-E-EL-H1976-192

Labprice Human PLAP/ALPP(Placental Alkaline Phosphatase) ELISA Kit

Elabscience Biotechnology

No significant cross-reactivity with analogues from other species was determined. Please, note that the data on cross-reactivity is limited. Other samples, aside from the tested ones, may be suitable to be used with this kit.

Description: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PLAP/ALPP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLAP/ALPP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLAP/ALPP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PLAP/ALPP. You can calculate the concentration of Human PLAP/ALPP in the samples by comparing the OD of the samples to the standard curve.

Target antigen: PLAP/ALPP
Species reactivity: Human
Sensitivity: 0.1ng/mL
Detection range: 0.16~10ng/mL: Assay type: Sandwich
Detection method: Colormetric
Tested sample types: Serum, Plasma, Cell supernatant

Shipped on ice packs. Upon receipt store refrigerated at +4 deg. C.

For research use only.