Cortisol ELISA KitElabscience Biotechnology
No significant cross-reactivity with analogues from other species was determined. Please, note that the data on cross-reactivity is limited. Other samples, aside from the tested ones, may be suitable to be used with this kit.
Description: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Cortisol. During the reaction, Cortisol in the sample or standard competes with a fixed amount of Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm Â± 2 nm. The concentration of Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Target antigen: Cortisol
Species reactivity: Universal
Detection range: 12.5~800ng/mL: Assay type: Competitive
Detection method: Colormetric
Tested sample types: Serum, Plasma, Cell supernatant
Shipped on ice packs. Upon receipt store refrigerated at +4 deg. C.
For research use only.