Labprice DNA Ligase4 Polyclonal AntibodyElabscience Biotechnology
Buffer composition: PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4.
Purified by: Affinity purification
Background: The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
Description: Rabbit antibody against Human DNA Ligase4 for WB,IHC-p,IF,ELISA applications.
Species reactivity: Human
Immunogen: Synthesized peptide derived from the Internal region of human DNA Ligase IV
Tested applications: WB,IHC-p,IF,ELISA
Application dilutions: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000
Shipped on ice packs. Upon receipt store at -20â„ƒ. Avoid freeze / thaw cycles.
Research Use Only.