Labprice Human IFN gamma Recombinant Protein

Description: Interferon-gamma (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Recombinant Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.

Gene name: IFN gamma
Host: E. coli
Label: None
Applications: Assay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 5.0-10.0 ng/ml. Assay #2: The ED50 was determined by a cytotoxicity assay using HT-29 cells is ? 0.05 ng/ml (> 2 x 107 units/mg).
Species Reactivity/Origin species: Monkey, Human, Mouse, Leech, Pig,
Purity: > 98% by SDS-PAGE & HPLC analyses
Endotoxin levels: < 0.1 ng/µg of protein (<1EU/µg)
Reconstitution Buffer: water
Buffer: 10mM Sodium Phosphate, pH 7.4
Additives/Stabilizer: None
Form: lyophilized
Storage: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C.
Reconstitution tips: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to ?80°C.
MW: 16.8 kDa
Length (aa): 144
Protein Sequence: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ
NCBI GeneID: 3458
Accession Number Protein: NP_000610.2
Accession Numberm RNA: NM_000619
Uniprot: P01579
Chromosomal location: 12q14

For research use only.